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Microwave Techniques and Protocols |
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From Book News, Inc.
Contributors from laboratories that are using microwaves as a means to facilitate biological sample processing offer a how-to manual for the clinical and research community. The methods and results they describe demonstrate that microwaves can be used routinely as the basis for improved sample processing for microscopy applications. They include complete sample processing protocols for light and electron microscopy, decalcification, and immunocytochemistry. Readers are assumed to have a solid background and experience in sample processing for those technologies. The black-and-white illustrations are excellent.Book News, Inc.
Book Info
Ted Pella Inc., Redding, CA. Comprehensive collection of time-saving microwave techniques, detailed instruction fo reproducible results, classic and current techniques for processing biological samples, and sample preparation for evaluation by different microscopic methods are all included.
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Preface Contributors 1 Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy 1 2 Vacuum-Assisted Microwave Processing of Animal Tissues for Electron Microscopy 13 3 Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy 25 4 Basic Procedure for Electron Microscopy Processing and Staining in Clinical Laboratory Using Microwave Oven 37 5 Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnostic Laboratory 49 6 Microwave Processing of Archived Pathology Specimens for Ultrastructural Examination 67 7 Microwave Fixation of Rat Hippocampal Slices 75 8 Microwave Processing Techniques for Biological Samples in a Service Laboratory 89 9 Microwave-Accelerated Decalcification: Useful Methods for Research and Clinical Laboratories 101 10 Microwave Processing of Sediment Samples 123 11 Microwave Polymerization in Thin Layers on London Resin White Allows Selection of Specimens for Immunogold Labeling 139 12 In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei 155 13 Microwave-Assisted Cytochemistry: Accelerated Visualization of Acetylcholinesterase at Motor Endplates 165 14 Microwave-Assisted Immunoelectron Microscopy of Skin: Localization of Laminin, Type IV Collagen, and Bullous Pemphigoid Antigen 173 15 Microwave Paraffin Techniques for Botanical Tissues 181 16 Microwave-Assisted Formalin Fixation of Fresh Tissue: A Comparative Study 191 17 Microwave-Assisted Processing of Biological Samples for Scanning Electron Microscopy 209 Index 217 |
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