|
|
|
ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¼ 3ÆÇ |
|
|
|
|
¡Ø±³È¯ ¹× ¹ÝÇ°¾È³» :
- ±³È¯À̳ª ¹ÝÇ°½Ã »óÇ° ¼ö·ÉÈÄ 3ÀÏÀ̳» ±³È¯À̳ª ¹ÝÇ° Àǻ縦 ¾Ë·ÁÁֽðí ÀÏÁÖÀÏÀ̳»¿¡ ÀúÈñÂÊÀ¸·Î »óÇ°ÀÌ ÀÔ°íµÇ¾î¾ßÇÕ´Ï´Ù. ½ÃÀÏÀÌ °æ°úµÇ¸é ¹ÝÇ°ÀÌ ºÒ°¡ÇÕ´Ï´Ù. - º¯½É¿¡ ÀÇÇÑ ±³È¯À̳ª ¹ÝÇ°½Ã, ¹è¼Ûºñ´Â °í°´´Ô²²¼ ºÎ´ãÇϽøç, ¿À¹è¼ÛÀ̳ª »óÇ° ºÒ·®½Ã¿¡´Â ¹«·á ±³È¯ÀÌ °¡´ÉÇÕ´Ï´Ù. - ¹ÝÇ°½Ã¿¡´Â ¹ÝÇ°»óÇ° °Ë¼öÈÄ Ä«µå Ãë¼Ò¸¦ Çص帮°Å³ª ÅëÀå,Àû¸³±ÝµîÀ¸·Î ȯºÒ Á¶Ä¡ ÇØ µå¸³´Ï´Ù. |
|
¡Ø¹è¼Û¾È³» :
- Á¦ÁÖµµ¹× µµ¼»ê°£Áö¿ªÀº Åùèºñ°¡ Ãß°¡µÉ¼ö ÀÖ½À´Ï´Ù. |
|
|
|
|
|
|
|
|
|
¡¡ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¼´Â ºÐÀÚ»ý¹°ÇÐÀ» °øºÎÇÏ´Â ¸¹Àº »ý¸í°øÇеµµé¿¡°Ô Á» ´õ ½±°Ô ±×¸®°í Ä£±Ù°¨ ÀÖ°Ô »ý¸íÇö»ó¿¡ Á¢±ÙÇÒ ¼ö ÀÖµµ·Ï °£°áÇÏ°í ü°èÀûÀ¸·Î Á¤¸®ÇÏ¿© ´Ù¾çÇÑ ½ÇÇèÁ¤º¸¸¦ ½±°Ô Á¢±ÙÇÒ ¼ö ÀÖµµ·Ï ¿«Àº ½ÇÇè¼ÀÌ´Ù. ½ÇÁ¦·Î ºÐÀÚ»ý¹°ÇÐ ½ÇÇèÀ» ÅëÇÏ¿© üÇèÇÏ°í Àǹ®Á¡À» °®°í Èï¹Ì¸¦ À¯µµÇϱâ À§ÇÏ¿© »ý¸í°øÇÐÀûÀÎ Çö»óÀÇ ÀÌÇظ¦ µ½±â À§ÇÑ °ÍÀÌ ºÐÀÚ»ý¹°ÇÐ ½ÇÇ輸¦ ÁýÇÊÇÏ°Ô µÈ µ¿±âÀÌ´Ù.
|
|
|
|
|
|
½ÇÇè 1 ¹ÚÅ׸®¾Æ ¹è¾ç ¹èÁö 32
½ÇÇè 2 Pure ¹ÚÅ׸®¾ÆÀÇ ºÐ¸® ¹× ¹è¾ç 41
½ÇÇè 3 ¼¼±Õ ¼öÀÇ ÃøÁ¤(ÆòÆÇ °è¼ö¹ý, Plate count technique) 47
½ÇÇè 4 ¹ÚÅ׸®¾Æ ¼¼Æ÷ÀÇ ¼ºÀå ¹× ±ÕÁÖ(strain) º¸°ü¹ý 53
½ÇÇè 5 Á¢ÇÕ(conjugation) 61
½ÇÇè 6 ¹ÚÅ׸®¾Æ ¼¼Æ÷·ÎºÎÅÍ plasmid DNAÀÇ extraction°ú purification 64
½ÇÇè 7 ÇÙ»ê°ú ´Ü¹éÁúÀÇ ³óµµ ÃøÁ¤ 73
½ÇÇè 8 Agarose gel electrophoresis 77
½ÇÇè 9 Agarose gel·ÎºÎÅÍ fragmented DNAÀÇ recovery ¹× Á¤Á¦ 83
½ÇÇè 10 Plasmid vector¸¦ ÀÌ¿ëÇÑ subcloning 87
½ÇÇè 11 Recombinant Plasmid DNAÀÇ ÇüÁúÀüȯ 94
½ÇÇè 12 ÇüÁúÀüȯüÀÇ °íµî»ý¹°Ã¼·ÎÀÇ ÇüÁúÀüȯ ¹× ºÐ¼® 100
½ÇÇè 13 ¥â-galactosidase enzymeÀÇ induction 113
½ÇÇè 14 Recombinant plasmid DNAÀÇ Å½Áö¿Í Á¦ÇÑÈ¿¼Ò Áöµµ ÀÛ¼º 117
½ÇÇè 15 ÁøÇÙ»ý¹°·ÎºÎÅÍ Genome DNAÀÇ ÃßÃâ 123
½ÇÇè 16 Eukaryotic DNAÀÇ partial restriction enzyme digestion 127
½ÇÇè 17 Southern Hybridization 130
½ÇÇè 18 In situ hybridization 136
½ÇÇè 19 ¹ýÀÇÇÐÀû Ãø¸é¿¡¼ÀÇ fingerprinting 143
½ÇÇè 20 ÁßÇÕÈ¿¼Ò ¿¬¼â¹ÝÀÀ(PCR, Polymerase Chain Reaction) 151
½ÇÇè 21 UV¿¡ ÀÇÇÑ DNA ¼Õ»ó ¹× ±¤È¸º¹ 155
½ÇÇè 22 °íµî»ý¹° Á¶Á÷À¸·ÎºÎÅÍ total RNAÀÇ extraction 159
½ÇÇè 23 mRNA(poly(A)+)ÀÇ Purification and selection 168
½ÇÇè 24 Northern hybridization 172
½ÇÇè 25 Dot and slot hybridization of RNA 180
½ÇÇè 26 Tyrosinase ´Ü¹éÁúÀÇ ÃßÃâ ¹× Á¤Á¦ 185
½ÇÇè 28 Tyrosinase È¿¼ÒÀÇ »ýÈÇÐÀû Ư¼º 192
½ÇÇè 28 Production of fusion protein (cloned genes) in E. coli 196
½ÇÇè 29 SDS-Polyacrylamide Gel electrophoresis 201
½ÇÇè 30 Western blotting 209
½ÇÇè 31 Translation of mRNA 218
½ÇÇè 32 Immunoprecipitation of target proteins 223
½ÇÇè 33 ELISA(Enzyme-Linked ImmunoSorvent Assay) ¹ÝÀÀ-È¿¼Ò¿¡°áÇÕÇÑ ¸é¿ªÈíÂøÁ¦ ºÐ¼®¹ý 229
½ÇÇè 34 plaque purification of Bacteriophage ¥ë 233
½ÇÇè 35 Single plaqueÀ¸·ÎºÎÅÍ bacteriophage ¥ëstockÀÇ Á¦Á¶ 237
½ÇÇè 36 Small-scale bacteriophage ¥ë liquid culture 241
½ÇÇè 37 Bacteriophage ¥ëDNAÀÇ Á¦Á¶ 244
½ÇÇè 38 bacteriophage ¥ëDNAÀÇ Á¦ÇÑÈ¿¼Ò¿¡ ÀÇÇÑ digestion 248
½ÇÇè 39 Bacteriophage ¥ëDNA armsÀÇ Á¤Á¦ 251
½ÇÇè 40 Bacteriophage ¥ëvectorÀÇ alkaline phosphatase È¿¼Ò·ÎÀÇ Ã³¸® 255
½ÇÇè 41 Bacteriophage ¥ëÀÇ foreign DNA fragment¿ÍÀÇ ligation and packaging 259
ºÎ·Ï 1 ºÐÀÚ»ý¹°ÇÐ °ü·Ã ¿ë¾× Á¦Á¶ 265
ºÎ·Ï 2 ºÐÀÚ»ý¹°ÇÐ ¹°ÁúÀÇ ´ÜÀ§µé 276
ºÎ·Ï 3 ¿ÏÃæ¿ë¾× 277
ºÎ·Ï 4 ¾Ð·ÂÀ» ¿Âµµ·Î ȯ»êÇÏ´Â ¹ý 280
ºÎ·Ï 5 Á¤»óÀÎÀÇ ¼Òº¯ ¼ººÐÇ¥(24½Ã°£´¢) 281
ºÎ·Ï 6 À¯¿ëÇÑ Web Sites 282
ºÎ·Ï 7 »ý¸í°øÇÐ Á¤º¸ Web Sites 285 |
|
|
|
|
|
|
|
|
µî·ÏµÈ ¼ÆòÀÌ ¾ø½À´Ï´Ù. |
|
|
|
|
|
|
|
|
|
|
|
|
|
|