Book Description
This second edition has been completely updated with new sections on molecular genetics and cancer research, reflecting the explosion of information for pathology techniques over the last 5 years. Covering general principles of ophthalmic pathology in a manageable length with over 900 illustrations - 50% in colour, this book allows for a clearer understanding. A bench book designed to support general pathologists who are required to provide a service in ophthalmic pathology and ophthalmologists who work in a service laboratory. The unconventional chapter outline is based on the nature of the specimens received in a laboratory e.g. The Traumatized Eye, The Conjunctival Biopsy. This gives the relatively inexperienced a quick lead into the methods required for specimen handling and the requirements for a pathological report by using available histopathological technology. Professor Lee is arguably the best known, most experienced and highly regarded UK Ophthalmic Histopathologist both in the UK and internationally. Superb illustrative material, much of which is in colour, makes this book invaluable to any trainee or practising pathologist with an interest in ophthalmic histopathology.

Book Info
Univ. of Glasgow, UK. Text covers general principles of ophthalmic pathology. Features chapter outlines based on the nature of specimens received in the laboratory. For ophthalmic histopathologists and general pathologists in the UK and internationally. Contains more than 900 halftone and color illustrations. Previous edition not cited. DNLM: Eye Diseases--pathology.

CHAPTER 1 EXAMINATION OF THE GLOBE. TECHNICAL ASPECTS: 1.1.1 Introduction 1.1.2 Clinical correlation 1.1.2.1 Background information 1.1.2.2 Clinical techniques for illustration and documentation 1.2 Equipment for macroscopic examination 1.3 Basic gross anatomy: External features 1.3.1 Measurement of ocular dimensions 1.3.2 The anterior segment 1.3.2.1 The cornea 1.3.2.2 The anterior chamber 1.3.2.3 The anterior sclera 1.3.3 The posterior aspect 1.3.3.1 The optic nerve 1.3.3.2 The vortex veins 1.3.4 Orientation of the primary cuts into the globe 1.3.4.1 Transillumination 1.3.4.2 Vertical calottes 1.3.4.3 Horizontal calottes 1.3.4.4 Oblique calottes 1.3.4.5 The unfixed globe 1.4 Basic gross anatomy: Internal features 1.4.1 Examination of specific features 1.4.2 Retrieval of tissue from the calottes 1.5 Radiological examination of the globe 1.6 Common artefacts 1.6.1 Myelin artefact 1.6.2 Shrinkage artefact 1.6.3. Autopsy material 1.7 Fixation techniques 1.7.1 Formalin based fixatives p11 1.7.1.1 Formaldehyde 1.7.1.2 Formal acetic methanol 1.7.2 Paraformaldehyde 1.7.3 Glutaraldehyde 1.8 Embedding techniques 1.8.1 General considerations 1.8.2 Paraffin wax 1.8.3 Celloidin 1.8.4 Plastic embedding 1.8.5 The importance of serial sections 1.9 Histological examination of a section of the globe 1.10 Specialised techniques 1.10.1 Introduction 1.10.2 Retinal digest preparations 1.10.3 Injection techniques (carbon, plastic) 1.11 Stains for microscopy 1.11.1 Conventional stains 1.11.2. Frozen sections for fat 1.11.3. Immunohistochemistry 1.11.3.1 Identification of tissue proteins by immune labelling 1.11.4 Silver stains for nucleolar organising regions (AgNOR's) 1.11.5 In situ hybridisation 1.11.6. Polymerase chain reaction 1.11.7. Techniques for identification of proliferation and apoptosis in cells. 1.12 Techniques for illustration and documentation 1.12.1 Photomacrography l.11.2 Photomicrography 1.12 Fluorescence microscopy 1.13 Polarised light/interference contrast microscopy