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Explores techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions, capillary electrophoresis (CE) analysis at high temperatures and the use of micro-CE and array-CE devices. Articles also cover techniques for quantitative estimation of gene compression using CE, and reviews practical choices for separation media and the CE environment DNA sequencing. Illustrated with diagrams and graphs.

Book Info

Univ. of Queensland, Australia. Reviews practical choices of separation media and the CE environment for DNA sequencing and includes techniques for the quantitative estimation of gene expression using CE. For basis and clinical investigators.

Preface v
Contents of Companion Volume xiii
Contributors xvii
Part I. Practical Applications for Rapid DNA Fragment Sizing and Analysis 1
1 Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis 3
2 Ultra-Fast DNA Separations Using Capillary Electrophoresis 19
3 Fast DNA Fragment Sizing in a Short Capillary Column 41
Part II. Practical Applications for Mutation Detection 47
4 Detection of DNA Polymorphisms Using PCR-RFLP and Capillary Electrophoresis 49
5 High Resolution Analysis of Point Mutations by Constant Denaturant Capillary Electrophoresis (CDCE) 57
6 Point Mutation Detection by Temperature-Programmed Capillary Electrophoresis 73
7 Single-Nucleotide Primer Extension Assay by Capillary Electrophoresis Laser-Induced Fluroescence 89
8 Amplification Refractory Mutation System Analysis of Point Mutations by Capillary Electrophoresis 95
9 SSCP Analysis of Point Mutations by Multicolor Capillary Electrophoresis 109
10 SSCP Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detector 127
11 Magnetic Bead-Isolated Single-Strand DNA for SSCP Analysis 135
Part III. Practical Applications for Genetic Analysis 149
12 Analysis of Short Tandem Repeat Markers by Capillary Array Electrophoresis 151
13 Genotyping by Microdevice Electrophoresis 163
14 Applications of Constant Denaturant Capillary Electrophoresis and Complementary Procedures: Measurement of Point Mutational Spectra 175
15 Toward Effective PCR-Based Amplification of DNA on Microfabricated Chips 191
16 Low-Stringency Single Specific Primer-Amplified DNA Analysis by Capillary Electrophoresis 205
17 DOP-PCR Amplification of Whole Genomic DNA and Microchip-Based Capillary Electrophoresis 211
18 Analysis of Triplet-Repeat DNA by Capillary Electrophoresis 221
19 Chemical Mismatch Cleavage Analysis by Capillary Electrophoresis with Laser-Induced Fluorescence Detection 231
Part IV. Practical Applications for Analysis of RNA and Gene Expression 241
20 Quantitiation of mRNA by Competitive PCR Using Capillary Electrophoresis 243
21 Quantitative RT-PCR from Fixed Paraffin-Embedded Tissues by Capillary Electrophoresis 253
22 Differential Display Analysis by Capillary Electrophoresis 259
Part V. Practical Applications for DNA Sequence Analysis 269
23 Capillary Array Electrophoresis Analyzer 271
24 DNA Sequencing at Elevated Temperature by Capillary Electrophoresis 289
25 DNA Sequencing in Noncovalently Coated Capillaries Using Low Viscosity Polymer Solutions 309
26 Selective Primer Sequencing from a DNA Mixture by Capillary Electrophoresis 317
27 Sequencing of RAPD Fragments Using 3'-Extended Oligonucleotide Primers 337
Part VI. Applications for Analysis of DNA-Protein and DNA-Ligand Interactions 345
28 Capillary Affinity Gel Electrophoresis 347
29 Capillary DNA-Protein Mobility Shift Assay 355
30 Protein-DNA Binding Affinities by Capillary Electrophoresis 369
31 Ligand Binding to Oligonucleotides 379
Index 393