Handbook of HLA Typing Techniques is an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. The information presented, much of it previously unpublished, was written by leading authorities in the field of transplantation. Each chapter provides detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

　Phenotyping topics covered include microlymphocytotoxicity assay, monoclonal antibodies, complement testing, serum screening for HLA antibodies, lymphocytotoxic crossmatching, and mixed and primed lymphocyte testing. Genotyping topics discussed include well-established RFLP analysis and polymerase chain reaction applications of PCR-SSO, PCR-RFLP, PCR-SSP, and PCR fingerprinting and related DNA heteroduplex technologies. Handbook of HLA Typing Techniques is an indispensable sourcebook for all researchers, practitioners, and students in the international tissue typing and human immunogenetics community.

Introduction

PCR-RFLP
HLA-DR and -DQ Typing by DNA-RFLP Analysis
Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies
A Comprehensive HLA-DRB, -DQB, and -DPB Oligotyping Procedure by Hybridization with Sequence-Specific Oligonucleotide Probes
HLA-DR Typing by Polymerase Chain Reaction Amplification with Sequence-Specific Primers (PCR-SSP)
Serological Typing of HLA-A, -B, and -C Antigens
HLA-DR and -DQ Serotyping
Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture
Definition of HLA-Dw and HLA-DPw Determinants by the Primed Lymphocyte Test

Appendix: HLA Class II Nucleotide Sequences, 1992
Index