Abstract
A collection of cutting-edge methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find reliable molecular protocols for the diagnosis of Fanconi anemia and dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and cheap methods for the detection of MRD in ALL and AML.

From Book News, Inc.
Researchers in pediatric hematology offer a collection of molecular techniques for the diagnosis, classification, and treatment of childhood hematological disorders. Each protocol is described in step- by-step detail, including the principle behind the method, required equipment, reagents, and known pitfalls. Methods include diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, the book details molecular human platelet antigen genotyping, a PCR procedure for thrombophilia screening, and methods for detecting residual leukemia below the threshold of light microscopy. The volume's 16 chapters are edited by Goulden and Steward, both of the Royal Hospital for Children in Bristol, UK.Copyright 2004 Book News, Inc., Portland, OR

Book Info
Royal Hospital for Children, Bristol, UK. Offers a collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders. Discusses the latest technology for the analysis of gene expression in childhood leukemia. Protocols are explained in detail.