For 40 years, Bancroft's Theory and Practice of Histological Techniques has established itself as the standard reference for histotechnologists and laboratory scientists, as well as histopathologists. With coverage of the full range of histological techniques used in medical laboratories and pathology departments, it provides a strong foundation in all aspects of histological technology - from basic methods of section preparation and staining, to advanced diagnostic techniques such as immunocytochemistry and molecular testing. This revised and updated 8th Edition by Kim S. Suvarna, Christopher Layton, and John D. Bancroft is a one-stop reference for all those involved with histological preparations and applications, from student to highly advanced laboratory professional.

New to this Edition
•Features new content on automation (computer logging and tracking, sub-sampling and archiving of samples), digital scanning techniques for slides used in remote/telepathology, and specialist molecular techniques.


•Provides superb visual guidance through the use of vivid color illustrations, including additional illustrations of diagnostic modalities and techniques.



•Contains more summary tables, charts, and boxes throughout for quick reference.



•Expert Consult™ eBook version included with purchase. This enhanced eBook experience allows you to search all of the text, figures, and references from the book on a variety of devices.


Key Features
•Presents a thorough, up-to-date, and detailed approach to the theory and protocols for preparing cells and tissues for laboratory evaluation, covering topics ranging from basic to advanced

Author Information
By Kim S Suvarna, MBBS, BSc, FRCP, FRCPath, Department of Pathology, Northern General Hospital, Sheffield; Christopher Layton, PhD, Specialist Section Lead in Specimen Dissection, Histopathology Department, Northern General Hospital, Sheffield, UK and John D. Bancroft, Formerly Pathology Directorate and Business Manager, Queen's Medical Center, Nottingham, UK

Pathology Laboratory Management


Chemical Safety in the Laboratory



Light Microscopy



Fixation of Tissues



The Gross Room



Tissue Processing



Microtomy



Resin Techniques



Theory of Histological Staining



The Hematoxylins and Eosin



Automation in the Histology Department



Connective and other Mesenchymal tissues with their stains



Carbohydrates



Pigments and minerals



Amyloid



Traditional stains and modern techniques for demonstrating Micro-organisms in Histology



Bone


18 Neuropathology and Muscle Biopsy Techniques

19 Immunohistochemical and Immunofluorescent Techniques

20 Molecular Pathology

21 Transmission Electron Microscopy

22 Digital Pathology


Appendices

Diagnostic Appendices

Appendix I Traditional Methods

Appendix II Tissue Microarray

Appendix III Applications of Immunohistochemistry


Technical Appendices

Appendix IV Measurement Units

Appendix V Preparation of Solutions

Appendix VI Buffer Solutions

Appendix VII Solubility of Some Common Reagents and Dyes

Appendix VIII Mounting Media and Slide Coatings